Improved contiguity of the threespine stickleback genome using long-read sequencing

نویسندگان

چکیده

Abstract While the cost and time for assembling a genome has drastically decreased, it still remains challenge to assemble highly contiguous genome. These challenges are rapidly being overcome by integration of long-read sequencing technologies. Here, we use improve contiguity threespine stickleback fish (Gasterosteus aculeatus) genome, prominent genetic model species. Using Pacific Biosciences sequencing, assembled freshwater from Paxton Lake. contigs this were able fill over 76.7% gaps in existing reference assembly, improving fivefold. Our gap filling approach was accurate, validated 10X Genomics long-distance linked-reads. In addition closing majority gaps, segments telomeres centromeres throughout This highlights power using long reads repetitive difficult regions genomes. latest build been released through newly designed community browser that aims consolidate growing number genomics datasets available fish.

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ژورنال

عنوان ژورنال: G3: Genes, Genomes, Genetics

سال: 2021

ISSN: ['2160-1836']

DOI: https://doi.org/10.1093/g3journal/jkab007